POLYMORPHISM OF ENDOSPERM PROTEINS IN AMPHIDIPLOIDS WITH THE G GENOME OF Triticum timopheevii (Zhuk.)
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Sep 1, 2016
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During evolution in Triticum the diversity of genes in T. aestivum L. was greatly reduced compared to its ancestors. This tendency restricted further improvement of productivity and quality in common wheat and narrowed the plant resistance to biotic and abiotic stresses. Wide hybridization resulted in synthetic genotypes that offered opportunities for introduction of new genes for useful traits in breeding. The objects of this study were two amphidiploids with G-genome inherited from tetraploid wheat relative T. timopheevii (2n=28, GGAuAu). Glutenin and gliadin allelic composition of the synthetic wheats H-68/44 and H-69/36 were analysed by SDS-PAGE and A-PAGE electrophoretic methods. New allelic variants in Glu-G1 loci, which are not characteristics for the spectrum of T. aestivum, were identified. In contrast to the high polymorphism of amphidiploids for high-molecular weight proteins, variation in the low-molecular glutenins was much less. More gliadin alleles in synthetic lines were found than in hexaploid wheat, due to the parent polymorphism. The results of this survey showed that synthetics with T. timopheevii genome might serve as an important sources of increased genetic variation for endosperm proteins in common wheat.
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1. Brown-Guedira, G., Gill, B., Cox, T., Leath S. (1997). Transfer of disease genes from Triticum araraticum to common wheat. Plant Breeding No. 116, pp. 105-112.
2. Ciaffi, M., Dominici, L., Lafiandra D. (1998). High molecular wight glutenin subunit variation in wild and cultivated einkorn wheats (Triticum ssp. Poaceae). Plant Syst. Evol. No. 209, pp. 123-137.
3. Gianibelli, M.C., Gupta, R.B., Lafiandra, D., Margiotta, B., MacRitchie F. (2001). Polymorphism of High Mr Glutenin Subunits in Triticum tauschii: Characterisation by chromatography and electrophoretic methods. Journal of Cereal Science No. 33, pp. 39-52.
4. Goncharov, N.P., Golovina, K.A., Kondratenko E.Y. (2009). Taxonomy and molecular phylogeny of natural and artificial wheat species. Breeding Science No. 59, pp. 492-498.
5. Gupta, R.B., Shepherd K.W. (1990). Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin.1. Varation and genetic control of the subunits in hexaploi wheats. Theor. Appl. Genet. No. 80, pp. 65–74.
6. Hu, X.G., Wu, B.H., Bi, Z., Liu, D.C., Zhang, L.Q., Yan, Z.H., Wei, Y.M., Zheng Y.L. (2012). Allelic variation and distribution of HMW glutenin subunit 1Ay in Triticum species. Genet. Resour Crop Evol. No. 59, pp. 491-497.
7. Jackson, E.A., Holt, L.M., Payne P.I. (1983). Characterization of high–molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and chromosomal localisation of their controlling genes. Theoretical and Applied Genetics No. 66, pp. 29-37.
8. Jackson, E.A., Morel, M.H., Sontag-Strohm, T., Branlard, G., Metakovsky, E.V., Redaelli R. (1996). Proposal for combining the classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat. Journal of Genetics and Breeding No. 50, pp. 321–336.
9. Jauhar, P.P., Peterson, T.S. (2006). Cytological analyses of hybrids and derivatives of hybrids between durum wheat and Thinopyrum bessarabicum, using multicolour fluorescent GISH. Plant Breeding No. 125, pp. 19 – 26.
10. Johansson, E., Henriksson, P., Svensson, G., Heneen W.K. (1993). Detection chromosome location and evalution of the functional value of a novel high Mr glutenin subunit found in Swedish wheats. J. Cereal Sci. No. 17, pp. 237-245.
11. Khan, K., McDonald, E., Banasik, O.J. (1983). Polyacrylamide gel electrophoresis of gliadin proteins for wheat variety. Identification-procedural modifications and observations. Cereal Chem. No. 60, pp. 178-181.
12. Laemmli, U.K. (1970). Clavage of structural proteins during the assembly of the head of bacteriofage T4. Nature No. 227, pp. 680 – 685.
13. Lafiandra, D., D’Ovidio, R., Porceddu, E., Margiotta, B., Colaprico G. (1993). New data supporting high Mr glutenin subunits as the determinant of quality differences among the pairs 5 + 10 vs. 2 + 12. Journal of Cereal Science No. 18, pp. 197-205.
14. Leonova, I., Budashkina, E., Kalinina, N., Röder, M., Börner, A., Salina E. (2011). Triticum aestivum-Triticum timopheevii introgression lines as a source of pathogen resistance genes. Czech. J. Genet. Plant. Breed. No. 47 (Special Issue), pp. S49-S55.
15. Li, B., You, M., Liang, R., Zhang, Y., Liu G. (2002). Polymorphism of high molecular weight glutenin subunits coded by G-genome. J. China Agric. Univ. No. 7, pp. 33-37.
16. Li, Q.Y., Yan, Y.M., Wang, A.L., An, X.L., Zhang, Y.Z., Hsam, S.L.K., Zeller, F.J. (2006). Detection of HMW glutenin subunit variation among 205 cultivated emmer accessions (Triticum turgidum ssp. dicoccum). Plant Breeding No. 125, pp. 120-124.
17. Li, X., Zhang, Y., Gao, L., Wang, A., Ji, K., He, Z., Appels, R., Ma, W., Yan Y. (2007). Molecular cloning, heterologous expression, and phylogenetic analysis of a novel y-type HMW glutenin subunit gene from the G genome of Triticum timopheevi. Genome No. 50, pp. 1130-1140.
18. Masci, S., Rovelli, L., Kasarda, D.D., Vensel, W.H., Lafiandra D. (2002). Characterisation and chromosomal localization of C-type low-molecular-weight glutenin subunits in the bread wheat cultivar Chinese Spring. Theoretical and Applied Genetics No. 104, pp. 422–428.
19. McIntosh, R., Yamazaki, Y., Dubcovsky, J., Rogers, J., Morris, C., Somers, D., Appels, R., Devos K. (2008). Catalogue of gene symbols for wheat. Available at http://www.shigen.nig.ac.jp/Wheat/komugi/genes
20. Monneveux, P., Zacharieva, M., Rekika (2000). The utilisation of Triticum and Aegilops species for the improvement of durum wheat. Options Mediterraneennes, Serie A: Seminares Mediterraneennes, № 40, pp. 71–81.
21. Mujeeb-Kazi, A. (2005). Wide crosses for durum wheat improvement. In: Durum wheat breeding – Current approaches and future strategies. C. Royo, M. Nachit, N. Di Fonzo, J.L. Araus, W. Pfeiffer, G. Slafer – Eds. Food Products Press, New York* London*Oxford, Vol. 2, pp.703- 745.
22. Obukhova, L., Budashkina, E., Shumni V. (2009). A study of the storage proteins in the introgression lines of common wheat (Triticum aestivum L. x Triticum timopheevii Zhuk.) resistant to brown leaf rust. Russian Journal of Genetics Vol.45, № 3, pp. 313-321.
23. Payne, P.I., Lawrence, G.J. (1983). Catalogue of alleles for the complex gene loci, Glu – A1, Glu – B1 and Glu – D1 which code for high-molecular-weight subunit in hexaploid wheat. Cereal Rresearch Communications No. 11(1), pp. 29-35.
24. Plamenov, D., Spetsov, P. (2011). Synthetic hexaploid lines are valuable resources for biotic stress resistance in wheat improvement. J. Plant Pathol. No. 93 (2), pp. 251-262.
25. Singh, N.K., Shepherd, K.W. (1988). Linkage mapping of genes controlling endosperm storage proteins in wheat endosperm. Theor. Appl. Genet. No. 71, pp. 79-92.
26. Singh, N.K., Shepherd, K.W, Cornish G.B. (1991). A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science No. 14, pp. 203-208.
27. Spetsov, P., Plamenov, D., Kiryakova V. (2006). Distribution and characterization of Aegilops and Triticum species from the Bulgarian Black Sea coast. Central European Journal of Biology No. 1(3), pp. 399-411.
28. Тоdorov, I. (2006). Study on seed proteins and their application as genetic markers in wheat breeding. D. Sci. dissertation, NCАN, Sofia.
29. Wan, Y., Wang, D., Shewry, P., Halford N. (2002). Isolation and characterization of the five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Ae. cylindrica. Theor. Appl. Genet. No. 104, pp. 828-839.
30. William, M.D.H.M., Peña, R.J., Mujeeb-Kazi A. (1993). Seed protein and isozyme variations in Triticum tauschii ( Aegilops squarrosa). Theor. Appl. Genet. No. 87, pp. 257-263.
31. Yan, Y.M., Hsam, S.L.K., Yu, J.Z., Jiang, Y., Zeller F.J. (2003). Genetic polymorphisms at Gli-Dt gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillyary electrophoresis. Plant Breeding No. 122, pp. 120-124.
32. Zaharieva, M., Dimov, A., Stankova, P., David, J., Monneveux P. (2003). Morphological diversity and potential interest for wheat improvement of three Aegilops L. species from Bulgaria. Genetic Resources and Crop Evolution, Vol. 50, № 5, pp. 56-61.
2. Ciaffi, M., Dominici, L., Lafiandra D. (1998). High molecular wight glutenin subunit variation in wild and cultivated einkorn wheats (Triticum ssp. Poaceae). Plant Syst. Evol. No. 209, pp. 123-137.
3. Gianibelli, M.C., Gupta, R.B., Lafiandra, D., Margiotta, B., MacRitchie F. (2001). Polymorphism of High Mr Glutenin Subunits in Triticum tauschii: Characterisation by chromatography and electrophoretic methods. Journal of Cereal Science No. 33, pp. 39-52.
4. Goncharov, N.P., Golovina, K.A., Kondratenko E.Y. (2009). Taxonomy and molecular phylogeny of natural and artificial wheat species. Breeding Science No. 59, pp. 492-498.
5. Gupta, R.B., Shepherd K.W. (1990). Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin.1. Varation and genetic control of the subunits in hexaploi wheats. Theor. Appl. Genet. No. 80, pp. 65–74.
6. Hu, X.G., Wu, B.H., Bi, Z., Liu, D.C., Zhang, L.Q., Yan, Z.H., Wei, Y.M., Zheng Y.L. (2012). Allelic variation and distribution of HMW glutenin subunit 1Ay in Triticum species. Genet. Resour Crop Evol. No. 59, pp. 491-497.
7. Jackson, E.A., Holt, L.M., Payne P.I. (1983). Characterization of high–molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and chromosomal localisation of their controlling genes. Theoretical and Applied Genetics No. 66, pp. 29-37.
8. Jackson, E.A., Morel, M.H., Sontag-Strohm, T., Branlard, G., Metakovsky, E.V., Redaelli R. (1996). Proposal for combining the classification systems of alleles of Gli-1 and Glu-3 loci in bread wheat. Journal of Genetics and Breeding No. 50, pp. 321–336.
9. Jauhar, P.P., Peterson, T.S. (2006). Cytological analyses of hybrids and derivatives of hybrids between durum wheat and Thinopyrum bessarabicum, using multicolour fluorescent GISH. Plant Breeding No. 125, pp. 19 – 26.
10. Johansson, E., Henriksson, P., Svensson, G., Heneen W.K. (1993). Detection chromosome location and evalution of the functional value of a novel high Mr glutenin subunit found in Swedish wheats. J. Cereal Sci. No. 17, pp. 237-245.
11. Khan, K., McDonald, E., Banasik, O.J. (1983). Polyacrylamide gel electrophoresis of gliadin proteins for wheat variety. Identification-procedural modifications and observations. Cereal Chem. No. 60, pp. 178-181.
12. Laemmli, U.K. (1970). Clavage of structural proteins during the assembly of the head of bacteriofage T4. Nature No. 227, pp. 680 – 685.
13. Lafiandra, D., D’Ovidio, R., Porceddu, E., Margiotta, B., Colaprico G. (1993). New data supporting high Mr glutenin subunits as the determinant of quality differences among the pairs 5 + 10 vs. 2 + 12. Journal of Cereal Science No. 18, pp. 197-205.
14. Leonova, I., Budashkina, E., Kalinina, N., Röder, M., Börner, A., Salina E. (2011). Triticum aestivum-Triticum timopheevii introgression lines as a source of pathogen resistance genes. Czech. J. Genet. Plant. Breed. No. 47 (Special Issue), pp. S49-S55.
15. Li, B., You, M., Liang, R., Zhang, Y., Liu G. (2002). Polymorphism of high molecular weight glutenin subunits coded by G-genome. J. China Agric. Univ. No. 7, pp. 33-37.
16. Li, Q.Y., Yan, Y.M., Wang, A.L., An, X.L., Zhang, Y.Z., Hsam, S.L.K., Zeller, F.J. (2006). Detection of HMW glutenin subunit variation among 205 cultivated emmer accessions (Triticum turgidum ssp. dicoccum). Plant Breeding No. 125, pp. 120-124.
17. Li, X., Zhang, Y., Gao, L., Wang, A., Ji, K., He, Z., Appels, R., Ma, W., Yan Y. (2007). Molecular cloning, heterologous expression, and phylogenetic analysis of a novel y-type HMW glutenin subunit gene from the G genome of Triticum timopheevi. Genome No. 50, pp. 1130-1140.
18. Masci, S., Rovelli, L., Kasarda, D.D., Vensel, W.H., Lafiandra D. (2002). Characterisation and chromosomal localization of C-type low-molecular-weight glutenin subunits in the bread wheat cultivar Chinese Spring. Theoretical and Applied Genetics No. 104, pp. 422–428.
19. McIntosh, R., Yamazaki, Y., Dubcovsky, J., Rogers, J., Morris, C., Somers, D., Appels, R., Devos K. (2008). Catalogue of gene symbols for wheat. Available at http://www.shigen.nig.ac.jp/Wheat/komugi/genes
20. Monneveux, P., Zacharieva, M., Rekika (2000). The utilisation of Triticum and Aegilops species for the improvement of durum wheat. Options Mediterraneennes, Serie A: Seminares Mediterraneennes, № 40, pp. 71–81.
21. Mujeeb-Kazi, A. (2005). Wide crosses for durum wheat improvement. In: Durum wheat breeding – Current approaches and future strategies. C. Royo, M. Nachit, N. Di Fonzo, J.L. Araus, W. Pfeiffer, G. Slafer – Eds. Food Products Press, New York* London*Oxford, Vol. 2, pp.703- 745.
22. Obukhova, L., Budashkina, E., Shumni V. (2009). A study of the storage proteins in the introgression lines of common wheat (Triticum aestivum L. x Triticum timopheevii Zhuk.) resistant to brown leaf rust. Russian Journal of Genetics Vol.45, № 3, pp. 313-321.
23. Payne, P.I., Lawrence, G.J. (1983). Catalogue of alleles for the complex gene loci, Glu – A1, Glu – B1 and Glu – D1 which code for high-molecular-weight subunit in hexaploid wheat. Cereal Rresearch Communications No. 11(1), pp. 29-35.
24. Plamenov, D., Spetsov, P. (2011). Synthetic hexaploid lines are valuable resources for biotic stress resistance in wheat improvement. J. Plant Pathol. No. 93 (2), pp. 251-262.
25. Singh, N.K., Shepherd, K.W. (1988). Linkage mapping of genes controlling endosperm storage proteins in wheat endosperm. Theor. Appl. Genet. No. 71, pp. 79-92.
26. Singh, N.K., Shepherd, K.W, Cornish G.B. (1991). A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science No. 14, pp. 203-208.
27. Spetsov, P., Plamenov, D., Kiryakova V. (2006). Distribution and characterization of Aegilops and Triticum species from the Bulgarian Black Sea coast. Central European Journal of Biology No. 1(3), pp. 399-411.
28. Тоdorov, I. (2006). Study on seed proteins and their application as genetic markers in wheat breeding. D. Sci. dissertation, NCАN, Sofia.
29. Wan, Y., Wang, D., Shewry, P., Halford N. (2002). Isolation and characterization of the five novel high molecular weight subunit of glutenin genes from Triticum timopheevi and Ae. cylindrica. Theor. Appl. Genet. No. 104, pp. 828-839.
30. William, M.D.H.M., Peña, R.J., Mujeeb-Kazi A. (1993). Seed protein and isozyme variations in Triticum tauschii ( Aegilops squarrosa). Theor. Appl. Genet. No. 87, pp. 257-263.
31. Yan, Y.M., Hsam, S.L.K., Yu, J.Z., Jiang, Y., Zeller F.J. (2003). Genetic polymorphisms at Gli-Dt gliadin loci in Aegilops tauschii as revealed by acid polyacrylamide gel and capillyary electrophoresis. Plant Breeding No. 122, pp. 120-124.
32. Zaharieva, M., Dimov, A., Stankova, P., David, J., Monneveux P. (2003). Morphological diversity and potential interest for wheat improvement of three Aegilops L. species from Bulgaria. Genetic Resources and Crop Evolution, Vol. 50, № 5, pp. 56-61.