ANTIBACTERIAL ACTIVITIES OF SOME WILD MUSHROOM EXTRACTS AGAINST PSEUDOMONAS AERUGINOSA

Article Sidebar

PDF
Published: Dec 1, 2018
Keywords:
Microdilution method, minimum bactericidal concentration (MBC).

Main Article Content

Daniela Nikolovska Nedelkoska
St. Kliment Ohridski University, Faculty of Technology and Technical Sciences, Veles, Republic of Maceonia
Natalija Atanasova-Pancevska
Ss. Cyril and Methodius University in Skopje, Faculty of Natural Science and Mathematics, Skopje, Republic of Macedonia
Mitko Karadelev
Ss. Cyril and Methodius University in Skopje, Faculty of Natural Science and Mathematics, Skopje, Republic of Macedonia
Zora Uzunoska
St. Kliment Ohridski University, Faculty of Technology and Technical Sciences, Veles, Republic of Macedonia
Dzoko Kungulovski
Ss. Cyril and Methodius University in Skopje, Faculty of Natural Science and Mathematics, Skopje, Republic of Macedonia

Abstract

Mushrooms synthesize a multitude of low-molecular-weight secondary metabolites that have an important role as communication signals, to defend mushroom habitat or to inhibit the growth of competitors. Investigations have shown that some of these metabolites have potent antimicrobial activity and could be beneficial for humans. In this study, antimicrobial potential of the extracts from six wild mushrooms: Amanita echinocephala, Russula medulata, Cerena unicolor, Hericium erinaceus, Ishnoderma benzoinum and Laetiporus sulphureus was evaluated against Gram-negative bacterium Pseudomonas aeruginosa. The antimicrobial activities of the methanolic mushroom extracts were investigated by the microdilution method. All the extracts that demonstrated inhibitory activities were further tested for bactericidal activity and minimum bactericidal concentration (MBC) values were determined. Antimicrobial activity was observed in all species included in the study, while the activities depended on the type and concentration of extract. The tested microorganism was more sensitive to the examined extracts from the polypore fungi (C. unicolor, H. erinaceus, I. benzoinum and L. sulphureus). The highest antibacterial activity was obtained in the extracts from polypores I. benzoinum and L. sulphureus (MBC=15.625 mg/mL). This study demonstrated that the analysed wild macrofungi have the potential to accumulate bioactive metabolites that possess antimicrobial activity.

Downloads

Download data is not yet available.

Article Details

Issue
Vol 72 No 3 (2018): Journal of Agricultural, Food and Environmental Sciences, JAFES
Section
Articles

References

Alves, M.J., Ferreira, I.C.F.R., Dias, J., Teixeira, V., Martins, A. and Pintado, M. (2012). A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Medica, 78 (16): 1707-1718.
2. Andrews, J. M. (2005). BSAC standardized disc susceptibility testing method (version 4). J Antimicrob Chemother, 56: 60-76.
3. Anke, T., Giannetti, B. and Steglich, W. (1982). Antibiotika aus Basidiomyceten, XV [1]. 1Hydroxy-2-nonin-4-on, ein antifungischer und cytotoxischer Metabolit aus Ischnoderma benzoinum (Wahl.) Karst. / Antibiotics from Basidiomycetes. XV [1]. 1-Hydroxy-2-nonyn-4-one, an Antifungal and Cytotoxic Metabolite from Ischnoderma benzoinum (Wahl.) Karst.. Zeitschrift für Naturforschung C, 37 (1-2): 1-4. doi:10.1515/znc-1982-1-201.
4. Moloney, M. G. (2016). Natural Products as a Source for Novel Antibiotics. Trends in Pharmacological Sciences, 37 (8): 689-701.
5. Nikolovska Nedelkoska, D., Atanasova-Pancevska, N., Karadelev, M. and Kungulovski, Dz. (2013). Screening of antibacterial and antifungal activities of selected Macedonian wild mushrooms. Matica Srpska Journal for Natural Sciences, 124: 333-340.
6. Nikolovska Nedelkoska, D., Atanasova-Pancevska, N., Karadelev, M. and Kungulovski, Dz. (2017). Bactericidal activities of selected macrofungi extracts against Staphylococcus aureus. Matica Srpska Journal for Natural Sciences, 133: 193-200.
7. Pala, S. A. and Wani, A. H. (2011). Mushrooms: The entities with multifarious medicinal properties. Journal of pharmacy research, 4 (12):4721-4726.
8. Poole, K. (2011). Pseudomonas Aeruginosa: Resistance to the Max. Frontiers in Microbiology, 2: 65.
9. Sarker, S. D., Nahar, L. and Kumarasamy, Y. (2007). Microtitre platebased antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 42: 321–324.
10. Suay, I., Arenal, F., Asensio, F. J., Basilio, A., Cabello, M. A., Díez, M. T., García, J. B., del Val, A. G., Gorrochategui, J., Hernández, P., Peláez F. and Vicente, M. F. (2000). Screening of basidiomycetes for antimicrobial activities. Antonie van 
Leeuwenhoek, 78: 129-39.
11. Teplyakova, T. V., Psurtseva, N. V., Kosogova, T. A., Mazurkova, N. A., Khanin, V. A. and Vlasenko, V. A. (2012). Antiviral activity of Polyporoid mushrooms (higher Basidiomycetes) from Altai Mountains (Russia). International Journal of Medicinal Mushrooms, 14 (1): 37–45.
12. Turkoglu, A., Duru, M. E., Mercan, N., Kivrak, I. and Gezer, K. (2007). Antioxidant and antimicrobial activities of Laetiporus sulphureus (Bull.) Murrill. Food Chem, 101: 267–273.
13. Yamac, M. and Bilgili, F. (2006). Antimicrobial activities of fruit bodies and/or mycelial cultures of some mushroom isolates. Pharm Biol, 44 (9): 660–667. 14. Zjawiony, J. K. (2004). Biologically active compounds from Aphyllophorales (polypore) fungi. J Nat Prod, 67 (2): 300-310.