SPECTROPHОTОMETRIC METHOD FOR DETERMINATION OF TOTAL POLYHENOLIC CONTENT IN SWEET CORN
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Dec 15, 2023
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Polyphenolic compounds also known as polyhydroxyphenols, are a group of secondary plant metabolites that can be found as simple molecules but also as highly polymerized compounds such as tannins. Many food types are considered as a great source of polyphenolic compounds, such as fruit, herbs, vegetables, grains and so on. Therefore, the goal of the present study was the determination of total polyphenolic content (TPC) in two sweet corn cultivars Messenger F1 and Sentinel F1 using UV – Vis spectroscopy. TPC in sweet corn are evaluated by Folin – Ciocâlteu method in relation with gallic acid as a reference standard. The obtained results reveled that the TPC in the hybrid Messenger F1 is
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Acosta-Estrada, B. A., Gutiérrez-Uribe, J. A., & Serna-Saldívar, S. O. (2014). Bound phenolics in foods, a review. Food chemistry, 152, 46-55.
Agbor, G. A., Vinson, J. A., & Donnelly, P. E. (2014). Folin – Ciocâlteu reagent for polyphenolic assay. International Journal of Food Science, Nutrition and Dietetics (IJFS), 3(8), 147-156.
Ainsworth, E. A., and Gillespie, K. M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols, 2(4), 875–877.
Daneshfar, A., Ghaziaskar, H. S., & Homayoun, N. (2008). Solubility of Gallic Acid in Methanol, Ethanol, Water, and Ethyl Acetate. Journal of Chemical & Engineering Data, 53(3), 776–778.
Dewanto, V., Wu, X., & Liu, R. H. (2002). Processed sweet corn has higher antioxidant activity. Journal of Agricultural and food Chemistry, 50(17), 4959-4964.
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Zhao, J., Khan, I. A., & Fronczek, F. R. (2011). Gallic acid. Acta Crystallographica Section E: Structure Reports Online, 67(2), o316-o317.
Harvey, D. (2000). Modern analytical chemistry. McGraw Hill.
Khoddami, A., Wilkes, M. A., & Roberts, T. H. (2013). Techniques for analysis of plant phenolic compounds. Molecules, 18(2), 2328-2375.
Lertrat, K., & Pulam, T. (2007). Breeding for increased sweetness in sweet corn. International Journal of Plant Breeding, 1(1), 27-30.
Matić, P., Sabljić, M., & Jakobek, L. (2017). Validation of spectrophotometric methods for the determination of total polyphenol and total flavonoid content. Journal of AOAC International, 100(6), 1795-1803.
Medina-Remón, A., Barrionuevo-González, A., Zamora-Ros, R., Andres-Lacueva, C., Estruch, R., Martínez-González, M. Á., ... & Lamuela-Raventos, R. M. (2009). Rapid Folin–Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake. Analytica Chimica Acta, 634(1), 54-60.
Musilova, J., Lachman, J., Bystricka, J., Polakova, Z., Kovacik, P., Hrabovska, D.(2013). The changes of the pоlyphenol content and antioxidant activity in potato tubers (Solanum tuberosum l.): Due to nitrogen fertilization. Vol.7.Potravinarstvo. No. 1, pp. 167-170.
Nichols, J. A., & Katiyar, S. K. (2010). Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Archives of dermatological research, 302, 71-83.
Popova, M., Bankova, V., Butovska, D., Petkov, V., Nikolova‐Damyanova, B., Sabatini, A. G., & Bogdanov, S. (2004). Validated methods for the quantification of biologically active constituents of poplar‐type propolis. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 15(4), 235-240.
Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine, 20(7), 933-956.
Shaghaghi, M., Manzoori, J. L., & Jouyban, A. (2008). Determination of total phenols in tea infusions, tomato and apple juice by terbium sensitized fluorescence method as an alternative approach to the Folin–Ciocalteu spectrophotometric method. Food chemistry, 108(2), 695-701.
Syedd-León, R., Orozco, R., Álvarez, V., Carvajal, Y., & Rodríguez, G. (2020). Chemical and antioxidant charaterization of native corn germplasm from two regions of Costa Rica: A conservation approach. International journal of food science, 2020.
Turati, F., Rossi, M., Pelucchi, C., Levi, F., & La Vecchia, C. (2015). Fruit and vegetables and cancer risk: a review of southern European studies. British Journal of Nutrition, 113(S2), S102-S110.
Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medicinal chemistry, 22(1), 132-149.
Zhang, R., Huang, L., Deng, Y., Chi, J., Zhang, Y., Wei, Z., & Zhang, M. (2017). Phenolic content and antioxidant activity of eight representative sweet corn varieties grown in South China. International journal of food properties, 20(12), 3043-3055.
Agbor, G. A., Vinson, J. A., & Donnelly, P. E. (2014). Folin – Ciocâlteu reagent for polyphenolic assay. International Journal of Food Science, Nutrition and Dietetics (IJFS), 3(8), 147-156.
Ainsworth, E. A., and Gillespie, K. M. (2007). Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols, 2(4), 875–877.
Daneshfar, A., Ghaziaskar, H. S., & Homayoun, N. (2008). Solubility of Gallic Acid in Methanol, Ethanol, Water, and Ethyl Acetate. Journal of Chemical & Engineering Data, 53(3), 776–778.
Dewanto, V., Wu, X., & Liu, R. H. (2002). Processed sweet corn has higher antioxidant activity. Journal of Agricultural and food Chemistry, 50(17), 4959-4964.
Jeftic. S. L., (1977). Kukuruz. „Nolit”. Beograd.
Zhao, J., Khan, I. A., & Fronczek, F. R. (2011). Gallic acid. Acta Crystallographica Section E: Structure Reports Online, 67(2), o316-o317.
Harvey, D. (2000). Modern analytical chemistry. McGraw Hill.
Khoddami, A., Wilkes, M. A., & Roberts, T. H. (2013). Techniques for analysis of plant phenolic compounds. Molecules, 18(2), 2328-2375.
Lertrat, K., & Pulam, T. (2007). Breeding for increased sweetness in sweet corn. International Journal of Plant Breeding, 1(1), 27-30.
Matić, P., Sabljić, M., & Jakobek, L. (2017). Validation of spectrophotometric methods for the determination of total polyphenol and total flavonoid content. Journal of AOAC International, 100(6), 1795-1803.
Medina-Remón, A., Barrionuevo-González, A., Zamora-Ros, R., Andres-Lacueva, C., Estruch, R., Martínez-González, M. Á., ... & Lamuela-Raventos, R. M. (2009). Rapid Folin–Ciocalteu method using microtiter 96-well plate cartridges for solid phase extraction to assess urinary total phenolic compounds, as a biomarker of total polyphenols intake. Analytica Chimica Acta, 634(1), 54-60.
Musilova, J., Lachman, J., Bystricka, J., Polakova, Z., Kovacik, P., Hrabovska, D.(2013). The changes of the pоlyphenol content and antioxidant activity in potato tubers (Solanum tuberosum l.): Due to nitrogen fertilization. Vol.7.Potravinarstvo. No. 1, pp. 167-170.
Nichols, J. A., & Katiyar, S. K. (2010). Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Archives of dermatological research, 302, 71-83.
Popova, M., Bankova, V., Butovska, D., Petkov, V., Nikolova‐Damyanova, B., Sabatini, A. G., & Bogdanov, S. (2004). Validated methods for the quantification of biologically active constituents of poplar‐type propolis. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 15(4), 235-240.
Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free radical biology and medicine, 20(7), 933-956.
Shaghaghi, M., Manzoori, J. L., & Jouyban, A. (2008). Determination of total phenols in tea infusions, tomato and apple juice by terbium sensitized fluorescence method as an alternative approach to the Folin–Ciocalteu spectrophotometric method. Food chemistry, 108(2), 695-701.
Syedd-León, R., Orozco, R., Álvarez, V., Carvajal, Y., & Rodríguez, G. (2020). Chemical and antioxidant charaterization of native corn germplasm from two regions of Costa Rica: A conservation approach. International journal of food science, 2020.
Turati, F., Rossi, M., Pelucchi, C., Levi, F., & La Vecchia, C. (2015). Fruit and vegetables and cancer risk: a review of southern European studies. British Journal of Nutrition, 113(S2), S102-S110.
Xie, Y., Yang, W., Tang, F., Chen, X., & Ren, L. (2015). Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current medicinal chemistry, 22(1), 132-149.
Zhang, R., Huang, L., Deng, Y., Chi, J., Zhang, Y., Wei, Z., & Zhang, M. (2017). Phenolic content and antioxidant activity of eight representative sweet corn varieties grown in South China. International journal of food properties, 20(12), 3043-3055.