HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR DETERMINATION OF PRESERVATIVES IN BEVERAGES
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Апстракт
Benzoic and sorbic acids, and their sodium, potassium and calcium salts are widely used as preservatives in acidic foods and beverages. They inhibit the growth of molds and yeasts, and are also effective against wide range of bacteria which explains the benefit of their usage. The health effects have led to limitation on the concentrations that can be used in food and beverages. Because of that, the analytical determination of these preservatives is important for consumer interest and protection. Therefore, a new reversed-phased HPLC method for a fast, simple, accurate and precise determination of potassium sorbate and sodium benzoate in different beverages is developed. In this purpose, a HPLC system equipped with UV diode array detection is employed. Separation and determination of investigated preservatives are performed using Purospher® STAR RP-18 (30 mm x 4 mm; 3 μm) analytical column. Methanol/phosphate buffer (pH = 3.70) (20/80, V/V) is used as a mobile phase, with flow rate of 1 mL/min, constant column temperature of 25°C and UV detection at 225 nm and 255 nm. Successful separation conditions are obtained using an isocratic elution within 6 min. Accuracy, precision, limit of detection, limit of quantification, and linearity range are evaluated. The separation factor value of 1.128 showed that this method can be successfully used for simultaneous
determination of benzoates and sorbates. The obtained data indicated that the concentration of investigated preservatives found in the analyzed beverages available on the local Macedonian markets is in accordance with Macedonian maximum levels for food safety.
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