PLUM POX VIRUS PRESENCE IN AUTOCHTHONOUS STONE FRUIT COLLECTION

Апстракт

Plum pox virus (PPV), also called Sharka belongs to Potyvirus genus, is the most devastating viral disease of stone fruit worldwide. Long distance spread is the result of moving infected nursery stock or propagative material, so grafts and budwood are also ways of moving infected material. Short distance spread is through aphid carriers. During 2024, a survey was performed to detect the presence of Plum pox virus (PPV) in stone fruits collection orchards in Aleksandrovac and at the site of Botanical Garden of the University of Banjaluka. In June, leaves plant tissues were collected and analyzed serologically by DAS-ELISA method with commercial antisera according to recommended protocol (Bioreba, Switzerland). A total of 38 accessions were analyzed where every sample include at least 2 trees. Plum, cherry, sour cherry, apricot and peach samples were collected, but all positive samples were from different accessions of plum trees. In total, 16 (42%) of symptom and symptomless plum accessions resulted as positive. The aim of this study was to observe the presence of Plum pox virus for further propagation and recovery procedures of autochthonous accessions of different stone fruits that exist in the collection of the Institute of Genetic Resources.