EFFECTS OF BOVINE PPARGC1A AND LTF GENE VARIANTS ON MILK YIELD AND COMPOSITION TRAITS IN HOLSTEIN-FRIESIAN AND JERSEY COWS
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Abstract
In this study, the association of bovine PPARGC1A and LTF gene polymorphisms with milk production and composition was investigated in dairy cattle. A total of 200 Holstein and Jersey cows, 100 from each breed, were used in the study. Total milk yield, 305-day, and test-day milk yield records were recorded. Milk fat/protein yield and percentage were calculated. Lactation rank, calving season, and service period were also taken into account in the analyses. Genomic DNA was extracted from whole blood samples by the phenol-chloroform-isoamyl alcohol method. Genotyping was carried out by the PCR-RFLP method. In this context, two polymorphisms at PPARGC1A and LTF genes located in intron 9 and 6, respectively, were evaluated. Genotypic/allelic frequencies, compliance with Hardy-Weinberg Equilibrium, and population genetics parameters were calculated. The general linear model (GLM) procedure was used to reveal the individual or interaction effects of these genes on the studied traits. The LTF/EcoRI marker was significantly associated with the lactation milk yield, 305-d milk yield, and 305-d milk fat yield in Jersey cattle. Moreover, the PPARGC1A×LTF interaction affected the test-d milk yield, test-d protein yield, 305-d milk yield, and 305-d milk fat yield in Jersey cattle. The CCAA and TTAB genotypes were found to be desirable for milk yield and fat content in Jersey cattle. The PPARGC1A×LTF interaction was also significantly associated with the test-d protein yield in the entire study population. This study may provide important knowledge on the genetic markers affecting milk production and the selection strategies in dairy cattle.
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