Main Article Content

Jasmina Ibrahimpašić
Vildana Jogić
Merima Toromanović
Aida Džaferović
Halid Makić
Samira Dedić


Recently, plant remediation techniques - phytoremediation - have been developed. Japanese Knotweed (Reynoutria japonica), is an invasive species with negative impacts on the environment and the economy. In order to assess the tolerance of highly invasive weeds to heavy metals, an experiment was conducted in which this plant was grown in control soil and in soils contaminated with different concentrations of Pb, Cd. The content of heavy metals in the soil did not eliminate the ability of Reynoutria japonica roots to regenerate. In soil contaminated with any concentration of Pb, the shoots of this plant grew at a similar rate as the control plants, and had the same morphological characteristics. Higher concentrations of cadmium and lead Cd (100, 200 mgkg-1), Pb (2000 mgkg-1) have extended rhizome regeneration compared to control plants. In soils contaminated with lower concentrations of Cd or Pb shoots grew at a similar rate as control plants. Chemical analysis of heavy metal content showed that this weed accumulated large amounts of metals when grown in soil contaminated with heavy metals A relatively high intake of Cd, aboveground plants. At a cadmium concentration of 100 mg kg-1, more than 537 times the amount of cadmium accumulated in the aboveground part of the plant, as opposed to that in control. The ability of Japanese Knotweed (Reynoutria japonica), to regenerate from parts of the rhizome, to grow and develop under stressful conditions in the presence of heavy metals indicates a high tolerance to heavy metals.

Key words: Japanese Knotweed (Reynoutria japonica), phytoremediation, heavy metal accumulation


Download data is not yet available.

Article Details



Alkorta, I., Hernández-Allica, J., Becerril, J., Amezaga, I., Albizu, I., Garbisu, C. (2004); Recent Findings on the Phytoremediation of Soils Contaminated with Environmentally Toxic Heavy Metals and Metalloids Such as Zinc, Cadmium, Lead, and Arsenic. Reviews in Environmental Science and Biotechnology, 3 (1), 71-90.
Angelova, R.V., Perifanova-Nemska, M.N.,Uzunova, G., Ivanov, K., Huu-Lee Q. (2016); Potential of Sunflower (Helianthus annuus L.) for Phytoremediation of Soils Contaminated with Heavy Metals. International Journal of Environmental and Ecological Engineering Vol (10), No.str.468-478.
Bailey J.P. (2003); Japanese Knotweeds s.l. at home and abroad. In: Child, J. H. Brock, G. Brundu, K. Prach, P. Pyšek, P.M. Wade, M. Williamson (eds.), Plant Invasions: Ecological Threats and Management Solutions, Backhuys Publishers, Leiden: 183–196.
Benyó D., Horváth E., Németh E., Leviczky T., Takács K., Lehotai N., Feigl G., Kolbert Z., Ördög A., Gallé R., Csiszár J., Szabados L. & Erdei L. (2016); Physiological and molecular responses to heavy metal stresses suggest different detoxification mechanism of Populus deltoides and P. × canadensis. Journal of Plant Physiology, 201, 62–70.
Benyó D., Horváth E., Németh E., Leviczky, T., Takács K., Lehotai N., Feigl G., Kolbert Z., Ördög A., Gallé R., Csiszár J., Szabados L., Erdei L.(2016); Physiological and molecular responses to heavymetal stresses suggest different detoxification mechanismof Populus deltoides and P. x canadensis. J. Plant Physiol.201, 62
Berchová-Bímová, K, J. Soltysiak, M. Vach. (2014); Role of different taxa and cytotypes in heavy metals absorption in Knotweeds (Fallopia), Scientia agriculturae bohemica, 45, (1): 11–18
Böhmoá P. & Šoltes R. (2017); Accumulation of selected element deposition in the organs of Fallopia
Bradley B.A., Blumenthal D.M., Wilcove D.S., Ziska L.H. (2010); Predicting plant invasions in an era of global change. Trends in Ecology and Evolution, 25, 310–318.
CABI. 2000. Invasive Species Compedium, Fallopia japonica (Japaneses Knotweed). [online].https://www.cabi.org/isc/datasheet/23875 (accessed: 11.05.2020) japonica during ontogeny. Oecologia Montana, 26, 35–46.
Michalet S., Rouifed S., Pellassa-Simon T., Fusade- Boyer M., Meiffren G., Nazaret S., Piola F.(2017); Tolerance of Japanese knotweed s.l. to soilartificial polymetallic pollution: early metabolic responses and performance during vegetative multiplication. Environ. Sci. Pollut. Res. Int., 24 (26), 20897–20907.
Mitić, V., Stankov- Jovanović, V., Ilić, M.D., Jovanović, S., Nikolić-Mandić, S. (2013). Uticaj požara na sadržaj teških metala u biljkama i zemljištu. Zaštita materijala 54, str. 75- 2.
Muthusaravanan S., Sivarajaseka N., Vivek, J.S, Paramasivan, T., Naushad Mu., Prakashmaran, J., Gayathri, V., Al‑Duaij O.K., (2018); Phytoremediation of heavy metals: mechanisms, methods and enhancements- Review, Environmental Chemistry Letters, https://doi.org/10.1007/s10311-018-0762-3
Nagajyoti P.C., Lee K.D. & Sreekanth T.V.M. (2010);Heavy metals, occurrence and toxicity for plants: A review. Environmental Chemistry Letters, 8, 199–216.
Nentwig W., Bacher S., Kumschick S., Pyšek P.,Vilà M. (2018); More than“100 worst”alien speciesin Europe. Biological Invasions, 20, 1611–1621, DOI: 10.1007/s10530–017–1651–6.
Newman, L., Reynolds, C. (2004); Phytodegradation of organic compounds. Current Opinion in Biotechnology, 15 (3), 225-230.
Nguyen, L.; (2002); The value of japanese knotweed in phytoremediation of contaminated soils along the Woonasquatucket river, Master Thesis, Brown University, Los Angeles
Novak N., Lodeta V., Kravarščan M. (2010); Japanese knotweed (Fallopia japonica (Houtt.) Ronse Decr.) - colonization in Croatia // 21st COLUMA Conference, International Meeting on Weed Control, Resumes. Dijon, Francuska, str. 58-58
Pravilnik o utvrđivanju dozvoljenih količina štetnih i opasnih tvari u zemljištu i metode njihovog ispitivanja, Službene novine Federacije BiH, 72/09, [Rulebook on Determining Permitted Quantities of Harmful and Dangerous Substances in Soil and Methods of Their Testing, Official Gazette of the Federation of BiH, 72/09]
Radojevic M. and Bashkin V.N. (2006); Practical Environmental Analysis. 2nd ed. United Kingdom, RSC Publishing.
Radojevic, M., Bashin, V. (1999); Practical Environmental Analzsis, Royal Society of Chemistry, Cambridge, UK.
Rahmonov O., Czylok A., Orczewska A., Majgier L. & Parusel T. (2014)a; Chemical composition of the leaves of Reynoutria japonica Houtt. and soil features in polluted areas, Central Europea Journal of Biology, 9, 320–330.
Rahmonov O., Banaszek B., Pukowiec-Kurda K. (2019)b; Relationships Between Heavy Metal Concentrations in Japanese Knotweed (Reynoutria Japonica Houtt.) Tissues and Soil in Urban Parks in Southern Poland. IOP Conf. Series: Earth and Environmental Science 221 012145 IOP. doi:10.1088/1755–1315 /221/1/012145.
Sharma, P. and Dubey, R.S. (2005); Lead Toxicity in Plants. Brazilian Journal of Plant Physiology, 17, 1-19. http://dx.doi.org/10.1590/s1677-04202005000100004 ..
Sołtysiak J., Berchová-Bímová K., Vach M., Brej T. (2011)a; Heavy metals content in the Fallopia genus in central European Cities – study from Wroclaw and Prague. Acta Botanica Silesiaca, 7, 209–218.
Sołtysiak J. and Brej T. (2014)b; Invasion of Fallopia genus plants in urban environment on the example of Wrocław city. Polish Journal of Environmental Studies, 23, 449–458.
Sołtysiak, J.,(2020)c; Heavy Metals Tolerance in an Invasive Weed (Fallopia japonica) under Different Levels of Soils Contamination, Journal of Ecological Engineering Vol. 21(7)
Takarina, N. D., Giok Pin Tjiong (2017); Bioconcentration Factor (BCF) and Translocation Factor (TF) of Heavy Metals in Mangrove Trees of Blanakan Fish Farm, Makara Journal of Science, 21/2, 77-8, doi: 10.7454/mss.v21i2.7308
Trinajstić, I. i sar.(1994); Prilog poznavanju rasprostranjenosti vrste Reynoutria japonica houtt. (Polygonaceae) u Hrvatskoj, Acta bot. croat. Vol. 53
Yasin M., Rosenqvist E., Jensen S.M. & Andreasen C.( 2019); The importance of reduced light intensity on the growth and development of six weed species, Weed Research, 59, 130–144.