HPLC МЕТОДИ ЗА КВАНТИТАТИВНО ОПРЕДЕЛУВАЊЕ НА АКТИВНАТА КОМПОНЕНТА ДАЗОМЕТ ВО ПЕСТИЦИДНАТА ФОРМУЛАЦИЈА BASAMID GRANULAT HPLC METHODS FOR QUANTITATIVE DETERMINATION OF DAZOMET IN PESTICIDE FORMULATION BASAMID GRANULAT

Abstract

For quantitative determination of dazomet in pesticide formulation Basamid Granulat, three HPLC methods are presented. Two methods are for reverse-phase liquid chromatography, and one for normal-phase liquid chromatography. The methods for reverse-phase determination of dazomet were performed on HS 3 x 3 C 8 (3,3 x 0,46 cm), LiChrosorb RP 8, (25 x 0,4 cm). For quantitative determination of dazomet with normal-phase liquid chromatography, LiChrosorb CN (25 x 0,4 cm), HS Pecosphere Silica 3 x 3 (3,3 x 0,46 cm) and LiChrosorb (Si 60 25  0,4 cm), columns were used. For validation of all develop methods, the evaluation is performed for: limit of detection, the linearity between the mass of analyte and peak area or peak height, intermediate precision of obtained results for retention time and peak area, percentage of recovered analyte and active ingredient quantity in pesticide formulation samples. The HS column type for quantitative determination of dazomet with reverse-phase liquid chromatography has some advantages: efficiency, precise, economic, and consists of small part of organic modifier in the mobile phase. The best value for limit of detection for dazomet 748,8 pg, was obtained on column type HS 3 X 3 C 8.