SPE-RRLC DETERMINATION OF SOME PESTICIDE RESIDUES IN APPLE JUICE
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Published:
Mar 1, 2016
Keywords:
RRLC determination, SPE, UV-DAD, pesticide residues, apple juice
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Abstract
This paper presents the development of RRLC (Rapid Resolution Liquid Chromatography) method for simultaneous determination of pesticides methomyl, methidathion and propiconazole in different clear apple juice samples. The experiments are performed using rapid resolution liquid chromatography system coupled with UV-VIS diode array detector. The developed high speed reversed-phase (RP) liquid chromatography method is carried out on the Purospher® Star RP-18 endcapped (30 mm × 4 mm; 3 μm) column, mobile phase consists of acetonitrille and water (50/50, V/V), flow rate of 1 mL/min, column temperature at 25 ºC and UV detection at 220 nm and 235 nm. Prior to RRLC analysis, the samples are cleaned up and concentrated using a solid-phase extraction (SPE). To assess the validity of the developed method, the following parameters are examined: selectivity, linearity, repeatability (precision), limit of detection, limit of quantification and accuracy.
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References
Albero, B., Sánchez-Brunete, C., Tadeo, J. L. (2003). Determination of organophosphorus pesticides in fruit juices by matrix solid-phase dispersion and gas chromatography. Journal of agricultural and food chemistry. No. 51(24), pp. 6915-6921.
Ay, R., Yasar, B., Demirözer, O., Aslan, B., Yorulmaz, S., Kaya, M., Karaca, I. (2007). The determination of the residue levels of some commonly used pesticides in Isparta apple orchards. Turkish Journal of Entomology. No. 31(4), pp. 297-306.
Carbo, L., Souza, V., Dores, E. F. G. C., Ribeiro, M. L. (2008). Determination of pesticide multiresidues in shallow groundwater in a cotton-growing region of Mato Grosso, Brazil. Journal of the Brazilian Chemical Society, Vol.19. No. 6, pp.1111-1117.
European Commission, Directorate General Health and Consumer Protection. (2010). Guidance document on pesticide residue analytical methods. pp. 9-11.
EPA. Registration Eligibility Decision (RED) for Methomyl. (1998). pp.14.
EPA. Registration Eligibility Decision (RED) for Methidathion. (2006a). pp.5.
EPA. Registration Eligibility Decision (RED) for Propiconazole. (2006b). pp. 5.
Guo, M., Tan, Z., Wu, H., Li, J., Wang, Y., Shi, J., Li, J., Jiang, L., Yao, X., Fang, L. (2012). Determination of 11 triazole fungicides in fruits using solid phase extraction and gas chromatography-tandem mass spectrometry. Chinese Journal of Chromatography. Vol. 30. No. 3, pp. 262-266.
He, Q., Li, J., Kong, X., Yue, A., Dong, H., Zhao, J. (2010). GC-MS Determination of Residual Amount of Propiconazole in Foodstuffs with Separation by Solid Phase Extraction. Physical Testing and Chemical Analysis. No. 2, pp. 178-183.
Hercegova, A., Mőder M. (2011). Determination of some selected pesticide residues in apple juice by solid-phase microextraction coupled to gas chromatography – mass spectrometry. Acta universitatis agriculturae et silviculturae mendelianae brunensis. Vol. LIX. No. 1, pp. 121-127.
http://ec.europa.eu/sanco_pesticides/public/index.cfm?event=activesubstance.selection
Jeannot, R., Sabik, H., Sauvard, E., Genin, E. (2000). Application of liquid chromatography with mass spectrometry combined with photodiode array detection and tandem mass spectrometry for monitoring pesticides in surface waters. Journal of chromatography A. No. 879, pp. 51-71.
Kadifkova-Panovska, T., Kavrakovski, Z., Bauer, S. (2000). Determination of propiconazole residues in tomatoes by gas chromatography. Bulletin of the Chemists and Technologists of Macedonia. Vol. 19. No.1, pp. 27-23.
Lagana, A., D'Ascenzo, G., Fago, G., Marino, A. (1997). Determination of organophosphorus pesticides and metabolites in crops by solid-phase extraction followed by liquid chromatography/diode array detection. Chromatographia. No. 46(5/6), pp. 256–264.
Nollet, L. (2000). Food Analysis by HPLC, Marcel Dekker Inc., New York, Basel, pp. 693-715.
Regulation (EC) No 396/2005 of the European Parliament and of the Council of 23 February 2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending Council Directive 91/414/EEC Text with EEA relevance.
Sánchez-Brunete, C., Albero, B., Tadeo, J. L., (2004). HPLC multiresidue method for the determination of N-methyl carbamates in fruit and vegetable juices. Journal of food protection No. 67 (11), pp. 2565-9.
SANCO. (2011). Method validation and quality control procedures for pesticide residues analysis in food and feed, Document Nº SANCO/12495/2011 (implemented by 01/01/2012). pp. 13.
Sannino, A., Bandini, M., Bolzoni, L. (1999). Multiresidue Determination of 19 Fungicides in Processed Fruits and Vegetables by Capillary Gas Chromatography after Gel Permeation Chromatography. Journal of AOAC International. Vol. 82. No. 5, pp. 1229-1238.
Schermerhorn, P., Golden, P. E., Krynitsky, A. J., Leimkuehler, W. M. (2005). Determination of 22 triazole compounds including parent fungicides and metabolites in apples, peaches, flour and water by Liquid Chromatography/Tandem Mass Spectrometry. Journal of AOAC International. Vol. 88. No. 5, pp. 1491-1502.
Stoytcheva, M., Zlatev, R. (2011). Pesticides in the Modern World - Trends in Pesticides Analysis. In Tech. pp. 1-526
Tomlin, C. (1997). The Pesticide Manual Incorporating the Agrochemicals Handbook. 11th Edition. Crop Protection Publications. pp. 675-676, 679-680, 855-857.
Yu, X., Wang, B. (2011). Rapid HPLC Analysis Method for Prochloraz and Propiconazole Content in Bactericidal Suspension Emulsion. Agrochemicals. No. 10, pp. 734-736.
Zamora, T., Pozo, O. J., López, F. J., Hernández, F. (2004). Determination of tridemorph and other fungicide residues in fruit samples by liquid chromatography-electrospray tandem mass spectrometry. Journal of Chromatography A. No. 1045(1-2), pp. 137-143.
Ay, R., Yasar, B., Demirözer, O., Aslan, B., Yorulmaz, S., Kaya, M., Karaca, I. (2007). The determination of the residue levels of some commonly used pesticides in Isparta apple orchards. Turkish Journal of Entomology. No. 31(4), pp. 297-306.
Carbo, L., Souza, V., Dores, E. F. G. C., Ribeiro, M. L. (2008). Determination of pesticide multiresidues in shallow groundwater in a cotton-growing region of Mato Grosso, Brazil. Journal of the Brazilian Chemical Society, Vol.19. No. 6, pp.1111-1117.
European Commission, Directorate General Health and Consumer Protection. (2010). Guidance document on pesticide residue analytical methods. pp. 9-11.
EPA. Registration Eligibility Decision (RED) for Methomyl. (1998). pp.14.
EPA. Registration Eligibility Decision (RED) for Methidathion. (2006a). pp.5.
EPA. Registration Eligibility Decision (RED) for Propiconazole. (2006b). pp. 5.
Guo, M., Tan, Z., Wu, H., Li, J., Wang, Y., Shi, J., Li, J., Jiang, L., Yao, X., Fang, L. (2012). Determination of 11 triazole fungicides in fruits using solid phase extraction and gas chromatography-tandem mass spectrometry. Chinese Journal of Chromatography. Vol. 30. No. 3, pp. 262-266.
He, Q., Li, J., Kong, X., Yue, A., Dong, H., Zhao, J. (2010). GC-MS Determination of Residual Amount of Propiconazole in Foodstuffs with Separation by Solid Phase Extraction. Physical Testing and Chemical Analysis. No. 2, pp. 178-183.
Hercegova, A., Mőder M. (2011). Determination of some selected pesticide residues in apple juice by solid-phase microextraction coupled to gas chromatography – mass spectrometry. Acta universitatis agriculturae et silviculturae mendelianae brunensis. Vol. LIX. No. 1, pp. 121-127.
http://ec.europa.eu/sanco_pesticides/public/index.cfm?event=activesubstance.selection
Jeannot, R., Sabik, H., Sauvard, E., Genin, E. (2000). Application of liquid chromatography with mass spectrometry combined with photodiode array detection and tandem mass spectrometry for monitoring pesticides in surface waters. Journal of chromatography A. No. 879, pp. 51-71.
Kadifkova-Panovska, T., Kavrakovski, Z., Bauer, S. (2000). Determination of propiconazole residues in tomatoes by gas chromatography. Bulletin of the Chemists and Technologists of Macedonia. Vol. 19. No.1, pp. 27-23.
Lagana, A., D'Ascenzo, G., Fago, G., Marino, A. (1997). Determination of organophosphorus pesticides and metabolites in crops by solid-phase extraction followed by liquid chromatography/diode array detection. Chromatographia. No. 46(5/6), pp. 256–264.
Nollet, L. (2000). Food Analysis by HPLC, Marcel Dekker Inc., New York, Basel, pp. 693-715.
Regulation (EC) No 396/2005 of the European Parliament and of the Council of 23 February 2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending Council Directive 91/414/EEC Text with EEA relevance.
Sánchez-Brunete, C., Albero, B., Tadeo, J. L., (2004). HPLC multiresidue method for the determination of N-methyl carbamates in fruit and vegetable juices. Journal of food protection No. 67 (11), pp. 2565-9.
SANCO. (2011). Method validation and quality control procedures for pesticide residues analysis in food and feed, Document Nº SANCO/12495/2011 (implemented by 01/01/2012). pp. 13.
Sannino, A., Bandini, M., Bolzoni, L. (1999). Multiresidue Determination of 19 Fungicides in Processed Fruits and Vegetables by Capillary Gas Chromatography after Gel Permeation Chromatography. Journal of AOAC International. Vol. 82. No. 5, pp. 1229-1238.
Schermerhorn, P., Golden, P. E., Krynitsky, A. J., Leimkuehler, W. M. (2005). Determination of 22 triazole compounds including parent fungicides and metabolites in apples, peaches, flour and water by Liquid Chromatography/Tandem Mass Spectrometry. Journal of AOAC International. Vol. 88. No. 5, pp. 1491-1502.
Stoytcheva, M., Zlatev, R. (2011). Pesticides in the Modern World - Trends in Pesticides Analysis. In Tech. pp. 1-526
Tomlin, C. (1997). The Pesticide Manual Incorporating the Agrochemicals Handbook. 11th Edition. Crop Protection Publications. pp. 675-676, 679-680, 855-857.
Yu, X., Wang, B. (2011). Rapid HPLC Analysis Method for Prochloraz and Propiconazole Content in Bactericidal Suspension Emulsion. Agrochemicals. No. 10, pp. 734-736.
Zamora, T., Pozo, O. J., López, F. J., Hernández, F. (2004). Determination of tridemorph and other fungicide residues in fruit samples by liquid chromatography-electrospray tandem mass spectrometry. Journal of Chromatography A. No. 1045(1-2), pp. 137-143.